Provis Biolabs
CAS No.83534-39-8
PNGase F Flash™ Cleavage Diagram

PNGase F Flash™

Rapid N-glycan removal enzyme for antibody analysis

Fastest N-Glycan Release

Optimized for high-throughput characterization, PNGase F Flash completes N-glycan removal in approximately 10 minutes

PNGase F Flash™ is a rapid deglycosylation protocol designed for time-critical and high-throughput analytical workflows. Maintaining the same high specificity and low protease activity as standard PNGase F, this accelerated formulation is perfect for rapid LC-MS peptide mapping, biosimilar characterization and rapid QC testing of mAbs.

Scientific Background

Catalyzes site-specific removal of N-linked oligosaccharides by hydrolyzing the asparagine-linked glycosylation site

Manufactured to high purity, providing excellent batch-to-batch consistency

Efficiently removes high-mannose, hybrid and complex N-glycans

Widely used in biopharmaceutical characterization and analytical biology workflows

PNGase Product Range

ParameterPNGase F
PNGase F Flash™
Primary FocusGeneral glycoprotein deglycosylation and structural analysisRapid antibody deglycosylation for high-throughput workflows
Reaction TimeStandard incubation tracking (2-16 hr.)Complete N-glycan removal in ~ 10 minutes
Applications
  • Glycoprotein analysis
  • Antibody deglycosylation
  • Rapid glycoprotein deglycosylation
  • High-throughput mAb analysis

Key Features

Complete N-Glycan Release in Under 10 Minutes
No Prior Protein Denaturation Step Required
High Throughput & Automation Compatible
Consistent Reproducible Results Across All Batches

Applications

Rapid glycoprotein deglycosylationHigh-throughput mAb/Fc glycan analysisLC-MS & peptide mappingBiosimilar characterizationQC testing