Complete Deglycosylation for Precise Glycan Analysis
PNGASE F ENZYMES FOR N-GLYCAN ANALYSIS
We offer two high-performance PNGase F enzymes designed to meet different deglycosylation needs. Standard PNGase F for routine workflows and PNGase F FlashTM for rapid, high-throughput applications.
OUR PNGASE F PRODUCT RANGE
PNGase F
SCIENTIFIC BACKGROUND
- Catalyzes site-specific removal of N-linked oligosaccharides by hydrolyzing the asparagine-linked glycosylation site.
- Manufactured to high purity, providing excellent batch-to-batch consistency.
- Efficiently removes high-mannose, hybrid, and complex N-glycans from native or denatured proteins.
- Widely used in biopharmaceutical characterization, including glycan profiling and protein mass determination.
KEY FEATURES
- Effectively removes most types of N-linked oligosaccharides from glycoproteins.
- Cleaves high-mannose, hybrid, and complex N-glycans with high specificity.
- Maintains integrity of the primary protein structure for downstream analysis.
- Enables high-confidence results in glycoprotein characterization and therapeutic protein analysis.
PNGase F Flash TM
SCIENTIFIC BACKGROUND
- Fast Acting Peptide-N-Glycosidase F used for rapid and efficient removal of N-linked glycans.
- Catalyzes cleavage of the asparagine-linked N-acetylglucosamine, enabling release of intact N-linked glycans.
- Optimized formulation enables complete deglycosylation in minutes.
- High purity recombinant production ensures specificity, consistency, and downstream compatibility.
KEY FEATURES
- Rapid deglycosylation of N-linked glycans in minutes, significantly reducing incubation time.
- Complete and unbiased removal of high-mannose, hybrid and complex N-glycans.
- Recombinant, animal-origin–free enzyme ensuring high purity and lot-to-lot consistency.
- Optimized formulation for high specificity, efficiency and reproducibility.
- Fully compatible with LC–MS, HPLC and intact mass analysis workflows.
COMPARISON
