Highly specific protease for efficient cleavage of C-terminal residues in protein processing
Carboxypeptidase B GMP-Grade
Scientific Background
- Carboxypeptidase B (CPB) (CAS Number 9025-24-5) is an enzyme that plays a crucial role in the field of protein analysis, specifically in the precise determination of protein sequences and post-translational modifications
- CPB is Zn²⁺ metalloprotease that hydrolyses basic amino acids L-Lys and L-Arg from the C-terminal position in polypeptides
- CPB consists of 307 amino acids with a molecular weight of 34.5 kDa and pl 5.76
- Recombinant CPB derived from recombinant DNA technology, offers enhanced performance, reliability, regulatory compliance and versatility for a wide range of biopharma applications
application
- Peptide mapping and protein sequencing
- Determination of protein C-terminal amino acid
- Sequence analysis by successive cleavage of C-terminal basic aminoacids
- Therapeutic insulin production by converting pro-insulin to insulin
- Therapeutic monoclonal antibodies manufacturing and quality control
Carboxypeptidase B
Scientific Background
- Carboxypeptidase B (CPB) (CAS Number 9025-24-5) is an enzyme that play a crucial role in the field of protein analysis, specifically in the precise determination of protein sequences and post-translational modifications
- CPB is Zn** metalloprotease that hydrolyses basic amino acids L-Lys and L-Arg from the C-terminal position in polypeptides
- CPB consists of 307 amino acids with a molecular weight of 34.5 kDa and pl 5.76
- Recombinant CPB derived from recombinant DNA technology, offers enhanced performance, reliability, regulatory compliance and versatility for a wide range of biopharma applications
Applications
- Peptide mapping and protein sequencing
- Determination of protein C-terminal amino acid
- Sequence analysis by successive cleavage of C-terminal basic amino baso animoacids
- Therapeutic insulin production by converting pro-insulin to insulin
- Therapeutic monoclonal antibodies manufacturing and quality control C Pep